Enhancing Muscle Intracellular Ca2+ Homeostasis and Glucose Uptake: Passive Pulsatile Shear Stress Treatment in Type 2 Diabetes

Type 2 diabetes mellitus (T2D) is a significant global public health problem that has seen substantial increase in the number of affected individuals recent decades. In murine model T2D (db/db), we found several abnormalities, including aberrant intracellular calcium concentration ([Ca2+]i), decreased glucose transport, increased production reactive oxygen species (ROS), elevated levels pro-inflammatory interleukins and creatine phosphokinase (CK), muscle weakness. Previously, demonstrated passive pulsatile shear stress, generated by sinusoidal (headward–forward) motion, using motion platform provides periodic acceleration whole body Z plane (pGz), induces synthesis nitric oxide (NO) mediated constitutive synthase (eNOS nNOS). We investigated effect pGz on db/db rodent T2D. The treatment mice with resulted beneficial effects. It reduced [Ca2+]i overload; enhanced transport; ROS levels, interleukins, CK. Furthermore, expression endothelial (eNOS), phosphorylated eNOS (p-eNOS), neuronal (nNOS); inducible (iNOS); improved strength. cytoprotective effects appear to be NO, since pretreatment L-NAME, nonspecific NOS inhibitor, abolished production. Our findings suggest non-pharmacological strategy such as therapeutic potential an adjunct